CST, 2224T, Caspase-2 (C2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Casp2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-2 (C2) Mouse Monoclonal Antibody (CST #2224) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Caspase-2 (C2) Mouse Monoclonal Antibody detects endogenous levels of procaspase-2 as well as its 14 and 12 kDa cleaved fragments. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal portion of human caspase-2. Background Caspase-2 (Nedd2/ICH-1) is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved at Asp316, producing a 14 kDa fragment and a 32 kDa prodomain/large subunit. Subsequent processing at Asp152 and Asp330 produces an 18 kDa large subunit and a 12 kDa small fragment (1). Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD (2). This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events (3, 4). In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase (5). Alternate Names CASP-2; CASP2; caspase 2; caspase 2 apoptosis-related cysteine peptidase; caspase 2, apoptosis-related cysteine peptidase; Caspase-2; Caspase-2 subunit p12; Caspase-2 subunit p13; Caspase-2 subunit p18; Caspase2; ICH-1 protease; ICH-1L; ICH-1L/1S; ICH1; NEDD-2; NEDD2; NEDD2 apoptosis regulatory; Neural precursor cell expressed developmentally down-regulated protein 2; neural precursor cell expressed, developmentally down-regulated 2; PPP1R57; Protease ICH-1; protein phosphatase 1, regulatory subunit 57 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 12, 14, 48 Source/Isotype: Mouse IgG1