CST, 2229S, Toll-like Receptor 2 Antibody

Polyclonal Antibody for studying TLR2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Toll-like Receptor 2 Antibody (CST #2229) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Toll-like Receptor 2 Antibody detects endogenous levels of total TLR2 protein. Cross reactivity was not detected with other family members at physiological conditions. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu179 of human TLR2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in , play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like/TIR-associated protein (MAL/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. Alternate Names CD282; TIL4; TLR2; toll like receptor 2; Toll-like receptor 2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 90-105 SOURCE: Rabbit