CST, 2258T, PU.1 (9G7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PU.1. Validated for WB,WB,IHC,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, PU.1 (9G7) Rabbit Monoclonal Antibody (CST #2258) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:200 - 1:800 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 45150 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), CUT&RUN Specificity / Sensitivity PU.1 (9G7) Rabbit Monoclonal Antibody detects endogenous levels of total PU.1 protein. This antibody does not cross react with other Ets family members. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PU.1 protein. Background PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells, including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells, and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF-4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8). Alternate Names 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-1 proto-oncogene; SPI-A; SPI1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG