CST, 2265S, Evi-1 Antibody

Polyclonal Antibody for studying EVI1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Evi-1 Antibody (CST #2265) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Evi-1 Antibody detects endogenous levels of total Evi-1 and MDS1/Evi proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Leu921 of human Evi-1. Antibodies are purified by protein A and peptide affinity chromatography. Background Evi-1 (Ecotropic virus integration site 1) was originally identified as a common site of viral integration in murine myeloid leukemia. It is involved in human myeloid disorders through chromosome translocation and inversion (1) and is also implicated in solid tumor formation (2). Evi-1 is a zinc finger transcription factor which also plays an important role in animal development (3). It has many isoforms due to alternative usage of 5'-ends (4), alternative splicing (5), and intergenic splicing which results in the formation of a fusion protein with MDS1 in normal tissues (6). Alternate Names AML1-EVI-1; AML1-EVI-1 fusion partner; AML1-EVI-1 fusion protein; ecotropic viral integration site 1; Ecotropic virus integration site 1 protein homolog; EVI-1; EVI1; Histone-lysine N-methyltransferase MECOM; KMT8E; MDS1; MDS1 and EVI1 complex locus; MDS1 and EVI1 complex locus protein; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MDS1-EVI1; MECOM; MGC163392; MGC97004; myelodysplasia syndrome 1 protein; myelodysplasia syndrome-associated protein 1; oncogene EVI1; PR domain 3; PRDM3; RUSAT2; zinc finger protein Evi1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105, 145, 200 SOURCE: Rabbit