CST, 2272S, Myc-Tag Antibody

Polyclonal Antibody for studying . Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Myc-Tag Antibody (CST #2272) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Myc-Tag Antibody detects recombinant proteins containing the Myc epitope tag. This antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody may cross-react with c-myc protein. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Antibodies are purified by protein A and peptide affinity chromatography. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only SOURCE: Rabbit