CST, 2285S, Thioredoxin 1 Antibody

Polyclonal Antibody for studying Thioredoxin 1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Thioredoxin 1 Antibody (CST #2285) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Thioredoxin 1 Antibody detects endogenous levels of total human thioredoxin 1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human thioredoxin 1. Antibodies are purified by protein A and peptide affinity chromatography. Background Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond that is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (TRX1) and mitochondrial thioredoxin 2 (TRX2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress, and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging, and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, because it acts as a negative regulator of the proapoptotic kinase ASK1 (3). Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5). Alternate Names ADF; ATL-derived factor; DKFZp686B1993; MGC61975; SASP; Surface-associated sulphydryl protein; testicular tissue protein Li 199; THIO; Thioredoxin; thioredoxin delta 3; TRDX; TRX; TRX1; TXN; TXN delta 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 12 SOURCE: Rabbit