CST, 2294T, DRAK2 (33D7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DRAK2. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, DRAK2 (33D7) Rabbit Monoclonal Antibody (CST #2294) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity DRAK2 (33D7) Rabbit Monoclonal Antibody detects endogenous levels of total DRAK2 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp315 of mouse DRAK2. Background DRAK2 (DAP kinase related apoptosis inducing protein kinase 2) is a member of the novel DAP (death associated protein) pro-apoptotic kinase family (1). Overexpression of DRAK2 in NIH/3T3 cells induces morphological changes associated with apoptosis, which are likely to occur in a p53-dependent manner (1,2). DRAK2 is preferentially expressed in lymphoid tissues and regulates the TCR activation threshold during thymocyte selection (3). Indeed, T cells from DRAK2 mice exhibit enhanced sensitivity to T cell receptor-mediated stimulation and have a reduced requirement for co-stimulation (4). Alternate Names DAP kinase-related apoptosis-inducing protein kinase 2; death-associated protein kinase-related 2; DRAK2; serine/threonine kinase 17b; Serine/threonine-protein kinase 17B; ST17B; STK17B Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG