CST, 2297S, 4E-T Antibody

Polyclonal Antibody for studying 4ET. Validated for Western Blotting. Highly specific and rigorously validated in-house, 4E-T Antibody (CST #2297) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity 4E-T Antibody detects endogenous levels of total 4E-T protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser949 of human 4E-T protein. Antibodies are purified by protein A and peptide affinity chromatography. Background During translation, eIF4E binds to the 5' cap structure of mRNA and helps mRNA bind to the ribosome. There are several factors that associate with eIF4E. One of these factors is eIF4E-Transporter (4E-T) (1). 4E-T has an eIF4E binding site, a nuclear localization signal and two nuclear export signals (1). Studies demonstrate that 4E-T functions as a nucleocytoplasmic shuttling protein mediating the import of eIF4E into the nucleus (1). Recent findings indicate that 4E-T, along with eIF4E, is concentrated in the processing bodies (P-bodies) in the cytoplasm (2). Furthermore, 4E-T decreases mRNA stability (2). Alternate Names 2610509L04Rik; 4E-T; 4E-transporter; 4ET; Clast4; eIF4E transporter; eIF4E-transporter; EIF4ENIF1; Eukaryotic translation initiation factor 4E nuclear import factor 1; Eukaryotic translation initiation factor 4E transporter; FLJ21601; FLJ26551 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 SOURCE: Rabbit