CST, 2302S, DARPP-32 Antibody

Polyclonal Antibody for studying DARPP-32. Validated for Western Blotting. Highly specific and rigorously validated in-house, DARPP-32 Antibody (CST #2302) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DARPP-32 Antibody detects endogenous levels of total DARPP-32 independent of phosphorylation. This antibody may also detect PP1. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr34 of human DARPP-32. Antibodies are purified by protein A and peptide affinity chromatography. Background DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3). Alternate Names DARPP-32; DARPP32; dopamine and cAMP regulated phosphoprotein; dopamine and cAMP-regulated neuronal phosphoprotein 32; Dopamine- and cAMP-regulated neuronal phosphoprotein; FLJ20940; PPP1R1B; PPR1B; protein phosphatase 1 regulatory inhibitor subunit 1B; Protein phosphatase 1 regulatory subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B (dopamine and cAMP regulated phosphoprotein, DARPP-32) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 32 SOURCE: Rabbit