CST, 23050S, CELA2A (E1D6G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CELA2A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CELA2A (E1D6G) Rabbit Monoclonal Antibody (CST #23050) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CELA2A (E1D6G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CELA2A protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly176 of human CELA2A protein. Background Chymotrypsin-like elastase family member 2A (CELA2A) was initially described as an exocrine elastase, produced by exocrine cells of the mammalian pancreas (1,2). Deeper examination revealed additional, lower levels of CELA2A expression by a small subset of pancreatic islet cells and many other tissues, including the adrenal glands, intestine and colon, liver, and white adipose tissue (3). Notably, gene expression datasets have reported aberrant CELA2A expression by other tissues in association with specific disease states (e.g., insulin resistance or Type II diabetes) (3), while increased expression of CELA2A by colonic epithelial cells was reported in patients with inflammatory bowel diseases (4). CELA2A is a secreted protease; in normal physiology, it is released into circulation following an elevation in plasma glucose levels, where it promotes both insulin secretion and degradation, leading to increased insulin sensitivity (3). Importantly, mutations in the gene were shown to inhibit the ability of CELA2A to regulate insulin dynamics (3). Collectively, these studies suggest that CELA2A may be an attractive therapeutic target in both metabolic and inflammatory diseases. Alternate Names AOMS4; CEL2A; CELA2A; chymotrypsin like elastase 2A; chymotrypsin like elastase family member 2A; Chymotrypsin-like elastase family member 2A; chymotrypsin-like elastase family, member 2A; ELA2A; elastase 2A; Elastase-2A; pancreatic elastase 2; pancreatic elastase IIA; PE-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG