CST, 2309S, gamma-Catenin Antibody

Polyclonal Antibody for studying Catenin-gamma. Validated for WB,IP,IHC,IF. Highly specific and rigorously validated in-house, gamma-Catenin Antibody (CST #2309) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity gamma-Catenin Antibody detects endogenous levels ot total γ-catenin protein. This antibody does not cross-react with β-catenin. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human γ-catenin. Antibodies are purified using protein A and peptide affinity chromatography. Background Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9). Alternate Names ARVD12; catenin (cadherin-associated protein), gamma (80kD); catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; CTNNG; Desmoplakin III; Desmoplakin-3; DP3; DPIII; gamma-catenin; Junction plakoglobin; JUP; PDGB; PKGB; PLAK Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 83 SOURCE: Rabbit