CST, 2312S, Synapsin Antibody

Polyclonal Antibody for studying Synapsin-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Synapsin Antibody (CST #2312) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Synapsin Antibody detects endogenous levels of total synapsin protein. This antibody is expected to recognize all synapsin isoforms. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser9 of human synapsin I. Antibodies are purified by protein A and peptide affinity chromatography. Background Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2). Alternate Names Brain protein 4.1; MRX50; SYN1; SYN1a; SYN1b; Synapsin; Synapsin I; synapsin Ib; Synapsin-1; SYNI Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 77 in mouse brain homogenate. SOURCE: Rabbit