CST, 23159S, Clusterin (E2O1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CLU mouse. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Clusterin (E2O1D) Rabbit Monoclonal Antibody (CST #23159) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:200 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity Clusterin (E2O1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total clusterin protein. This antibody cross-reacts with a nonspecific band at 250 kDa. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val271 of mouse clusterin protein. Background Clusterin (CLU, apolipoprotein J) is a multifunctional glycoprotein that is expressed ubiquitously in most tissues. Clusterin functions as a secreted chaperone protein that interacts with and stabilizes stress-induced proteins to prevent their precipitation (1,2). Research studies show that clusterin plays a protective role in Alzheimer's disease by sequestering amyloid β(1-40) peptides to form long-lived, stable complexes, which prevents amyloid fibril formation (3-5). In addition to the secreted protein, several intracellular isoforms are localized to the nucleus, mitochondria, cytoplasm, and ER. The subcellular distribution of these multiple isoforms leads to the diversity of clusterin functions. Additional studies report that clusterin is involved in membrane recycling, cell adhesion, cell proliferation, apoptosis, and tumor survival (6-9). The clusterin precursor is post-translationally cleaved into the mature clusterin α and clusterin β forms. Clusterin α and β chains create a heterodimer through formation of disulfide bonds (10). Alternate Names AI893575; Apo-J; Apoj; Apolipoprotein J; Cli; Clu; CLUS; Clusterin; Clusterin alpha chain; Clusterin beta chain; Clustrin; complement lysis inhibitor; D14Ucl; D14Ucla3; Msgp-2; Sgp; SGP-2; Sgp2; SP-; SP-40; Sugp; Sugp-2; Sulfated glycoprotein 2; testosterone repressed prostate message; testosterone repressed prostate message-2 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 32-42, 65, 75 Source/Isotype: Rabbit IgG