CST, 23160W2, HA-Tag (6E2) Mouse Monoclonal Antibody (SignalFlex™ Alexa Fluor® 555 Conjugate)

Monoclonal Antibody for studying . Highly specific and rigorously validated in-house, HA-Tag (6E2) Mouse Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 555 Conjugate) (CST #23160) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity HA-Tag (6E2) Mouse mAb (SignalFlex™ Alexa Fluor Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) . Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been used extensively as a general epitope tag in expression vectors (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1