CST, 2316T, CDK9 (C12F7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cdk9. Validated for WB,IP,IHC,IF,F,C&R,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, CDK9 (C12F7) Rabbit Monoclonal Antibody (CST #2316) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 CUT&RUN: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 66968 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), CUT&RUN Specificity / Sensitivity CDK9 (C12F7) Rabbit Monoclonal Antibody detects endogenous levels of total CDK9 protein, both 42 kDa and 55 kDa isoforms. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine, Dog Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CDK9. Background P-TEFb is a general transcription factor that regulates transcription elongation through phosphorylation of the C-terminal tail domain (CTD) of RNA polymerase II (RNAP II). The P-TEFb complex is composed of a catalytic subunit, CDK9, and its regulatory cyclin partner, which can be cyclin T1, T2a, T2b or K (reviewed in 1,2). P-TEFb is recruited by the HIV Tat protein to allow transcriptional elongation, and subsequent replication of the viral genome. Inhibition of P-TEFb function therefore has potential for HIV therapy. CDK9 exists as two isoforms, an abundant 42 kDa isoform, and a less abundant 55 kDa isoform, which contains an amino-terminal extension (3). The two forms likely have distinct purposes based on differential expression during lymphocyte activation (4,5) and on their localization within the nucleus (5).Cyclin dependent kinases (CDKs) are activated in part by cyclin binding and by phosphorylation of a conserved threonine in the T-loop domain. Phosphorylation of CDK9 at the T-loop Thr186 by an unidentified nuclear kinase may be important in P-TEFb activation (6) and regulation of HIV transcription (7). Acetylation of CDK9 at Lys44 affects its ability to phosphorylate the RNAPII CTD (8). Alternate Names C-2K; CDC2-related kinase; CDC2L4; CDK9; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; CTK1; cyclin dependent kinase 9; Cyclin-dependent kinase 9; PITALRE; PTEFB; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK; Tat-associated kinase complex catalytic subunit Specification REACTIVITY: H M R Hm Mk B Dg SENSITIVITY: Endogenous MW (kDa): 42, 55 Source/Isotype: Rabbit IgG