CST, 23177S, OGT (D1D8Q) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying OGT. Validated for Western Blotting. Highly specific and rigorously validated in-house, OGT (D1D8Q) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #23177) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity OGT (D1D8Q) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of total OGT protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro546 of human OGT protein. Background O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis, and transcription (2,3), and research studies have implicated this modification in cancer (1). The reversible protein modification by O-GlcNAc, which has been suggested to be a nutrient and stress sensor, is catalyzed by two highly conserved enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) (4). Alternate Names FLJ23071; HINCUT-1; HRNT1; MGC22921; MRX106; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine (GlcNAc) transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; OGT; OGT1; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG