CST, 23200S, p16 INK4A (F2T7H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p16-INK4A mouse. Validated for WB,WB,IP,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, p16 INK4A (F2T7H) Rabbit Monoclonal Antibody (CST #23200) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:1600 Immunofluorescence (Immunocytochemistry): 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 44872 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity p16 INK4A (F2T7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total p16 INK4A protein. This antibody does not cross-react with p15 INK4B protein. Non-specific cytoplasmic staining was observed in mouse small intestine epithelium by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length mouse p16 INK4A protein. This antibody recognizes residues surrounding Arg156 of mouse p16 INK4A protein. Background Members of the INK4 family of cyclin-dependent kinase inhibitors include p16 INK4A, p15 INK4B, p18 INK4C, and p19 INK4D. The INK4 family members inhibit cyclin-dependent kinases 4 and 6 (CDK4 and CDK6), causing cell cycle arrest in G1 phase. The INK4A-ARF-INK4B locus on chromosome 9p21, frequently lost in human cancer, encodes the INK4 family members p16 INK5A and p15 INK4B, as well as the unrelated protein, ARF (1). p16 INK4A expression, typically repressed in the absence of stress, is thought to drive cells into senescence, and p16 INK4A expression is a commonly used marker of senescent cells (2,3). p16 INK4A protein expression is often altered in human cancer, and high expression is currently used as a predictive biomarker in cervical cancer (3-5). Alternate Names alternative reading frame; Arf; ARF-; ARF-INK4a; CD2A1; CDK4I; Cdkn2a; CDN2A; cyclin dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A, isoform 3; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a-ARF; Ink4a/Arf; mitochondrial smARF; MTS; MTS1; OTTMUSP00000008143; p16; p16-INK4; p16-INK4a; p16(INK4a); p16I; P16ink4a; p19; p19; p19ARF; Pct; Pctr1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 16 Source/Isotype: Rabbit IgG