CST, 2332S, eEF2 Antibody

Polyclonal Antibody for studying eEF2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, eEF2 Antibody (CST #2332) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity eEF2 Antibody detects endogenous levels of total eEF2 independent of phosphorylation. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino-terminus of human eEF2. Antibodies are purified by protein A and peptide affinity chromatography. Background Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity (1-4). eEF2 kinase is normally dependent on Ca2+ ions and calmodulin (5,6). eEF2 kinase can also be activated by PKA in response to elevated cAMP levels (7-9), which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A) (10). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both (11). Alternate Names EEF-2; EEF2; EF-2; EF2; Elongation factor 2; epididymis secretory sperm binding protein; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase; SCA26 Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous MW (kDa): 95 SOURCE: Rabbit