CST, 23368T, Cathepsin Z (F6D7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CTSZ. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Cathepsin Z (F6D7U) Rabbit Monoclonal Antibody (CST #23368) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Cathepsin Z (F6D7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total cathepsin Z protein. Non-specific staining was observed in human salivary gland and in cardiomyocytes by immunohistochemistry. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn75 of human cathepsin Z protein. Background Cathepsin Z, also known as cathepsin X or cathepsin P, is a lysosomal cysteine proteinase member of the papain family encoded by the gene (1,2). It contains a uniquely short pro-domain containing an RGD integrin binding sequence, allowing functionality in cell adhesion, signaling, and extracellular matrix remodeling (3,4). Cathepsin Z is expressed in immune cells, specifically macrophages, monocytes, and dendritic cells, where it participates in several immune cell functions (5). It may play a role in tumorigenesis and has been implicated in several cancer types, including hepatocellular carcinoma, glioblastoma, non-small cell lung cancer, prostate cancer, and colorectal cancer (6-10). Cathepsin Z is also expressed and secreted by activated microglia in the brain and may play a role in neuroinflammation (11,12). Alternate Names carboxypeptidase LB; cathepsin B2; cathepsin IV; Cathepsin P; Cathepsin X; cathepsin Y; Cathepsin Z; cathepsin Z1; CATZ; CTSX; CTSZ; cysteine-type carboxypeptidase; FLJ17088; lysosomal carboxypeptidase B; preprocathepsin P Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG