CST, 2339S, p38 beta MAPK (C28C2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p38-beta MAPK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, p38 beta MAPK (C28C2) Rabbit Monoclonal Antibody (CST #2339) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity p38 beta MAPK (C28C2) Rabbit Monoclonal Antibody detects endogenous levels of total p38β MAPK protein. This antibody does not cross-react with other isoforms of p38 MAPK. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around the carboxy terminus of p38β MAPK. Background p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). Although there are many similarities between the four p38 isoforms, there are also some important differences that suggest that the various members may regulate specific functions, and the presence of multiple p38 isoforms may provide a mechanism for the generation of tissue-specific or stimulus-specific responses to the activation of the p38 signal transduction pathway (9,10). Alternate Names MAP kinase 11; MAP kinase p38 beta; MAPK 11; MAPK11; Mitogen-activated protein kinase 11; Mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; MK11; p38-2; p38b; p38Beta; P38BETA2; PRKM11; SAPK2; SAPK2B; Stress-activated protein kinase 2; Stress-activated protein kinase 2b; stress-activated protein kinase-2; stress-activated protein kinase-2b Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG