CST, 23428S, Human Activated T Cell Markers Flow Cytometry Panel

Assay Kit for studying CD3epsilon/CD69/IL2RA/CD8A/CD4 in the research area. Product Usage Information All antibodies in this kit are compatible with the Flow Cytometry, Live Cell Protocol for Directly Conjugated Antibodies and can be used in a single staining mix. After antibody staining and prior to acquisition on a flow cytometer, we recommend adding Propidium Iodide or 7-AAD to enable identification and exclusion of dead cells from the analysis. Gating strategy for identifying activated T cells: If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Activated helper T cells are positive for expression of CD69 and/or IL-2Rα/CD25. Regulatory T cells can also express IL-2Rα/CD25. Cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Activated cytotoxic T cells are positive for expression of CD69 and/or IL-2Rα/CD25. Storage Antibodies are supplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH7.2. Store at 4° C. Do not aliquot the antibodies. Protect from light. Do not freeze. All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific "Best By" dates for each individual component. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity Each antibody in the Human Activated T Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein. CD3 (UCHT1) Mouse Monoclonal Antibody (PE Conjugate), CD4 (RPA-T4) Mouse Monoclonal Antibody (violetFluor 450 Conjugate), CD8 alpha (RPA-T8) Mouse Monoclonal Antibody (FITC Conjugate), IL2-R alpha/CD25 (BC96) Mouse Monoclonal Antibody (APC Conjugate), and CD69 (FN50) Mouse Monoclonal Antibody (PE-Cy7 Species Reactivity: Human Source / Purification Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.