CST, 2342S, Dynamin 1/2 Antibody

Polyclonal Antibody for studying DYN1/DYN2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Dynamin 1/2 Antibody (CST #2342) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:30 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Dynamin 1/2 Antibody detects endogenous levels of total dynamin protein. This antibody does not recognize the related dynamin-like protein DLP. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr231 of human dynamin. Antibodies are purified by protein A and peptide affinity chromatography. Background Dynamin is a family of large GTPases that has been implicated in the formation of vesicles of both the endocytotic and secretory processes (1). Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the response to extracellular signals. It has been illustrated that dynamin interacts with signaling proteins such as Src, PLCγ, PKC and G-proteins. PKC and Src phosphorylate dynamin, and its phosphorylation may regulate the endocytosis of cell surface receptors (2,3). Alternate Names CMT2M; CMTDI1; CMTDIB; DI-CMTB; DNM; DNM1; DNM2; DYN1; DYN2; dynamin 1; dynamin 2; dynamin II; Dynamin-1; Dynamin-2; DYNII; EIEE31; LCCS5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit