CST, 23499T, POMC (D3R1U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying POMC. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, POMC (D3R1U) Rabbit Monoclonal Antibody (CST #23499) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity POMC (D3R1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total POMC protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro69 of human POMC protein. Background Pro-opiomelanocortin (POMC) is a precursor protein expressed in the pituitary and the brain where it is processed into several peptide hormones and neuropeptides. Among these peptides are ACTH, α- and β-MSH, β-and γ-LPH, CLIP, β-endorphin, and N-POMC (1). POMC is involved in hypothalamic circuits regulating feeding behavior and POMC-producing neurons promote satiety (2). POMC neurons are also the target of leptin and insulin for the promotion of the browning of white fat (3). Alternate Names ACTH; Adrenocorticotropic hormone; adrenocorticotropin; alpha-melanocyte-stimulating hormone; Alpha-MSH; Beta-endorphin; Beta-LPH; beta-melanocyte-stimulating hormone; Beta-MSH; CLIP; COLI; Corticotropin; Corticotropin-like intermediary peptide; Corticotropin-lipotropin; Gamma-LPH; Gamma-MSH; Lipotropin beta; Lipotropin gamma; LPH; Melanocyte-stimulating hormone alpha; Melanocyte-stimulating hormone beta; Melanotropin alpha; Melanotropin beta; Melanotropin gamma; Met-enkephalin; MSH; NPP; OBAIRH; opiomelanocortin prepropeptide; POC; POMC; Potential peptide; pro-ACTH-endorphin; Pro-opiomelanocortin; proopiomelanocortin; proopiomelanocortin preproprotein Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 10, 12, 28, 32 Source/Isotype: Rabbit IgG