CST, 23522S, SMUG1 (E7P4I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMUG1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SMUG1 (E7P4I) Rabbit Monoclonal Antibody (CST #23522) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SMUG1 (E7P4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMUG1 protein. Based on the sequence of the petide antigen, this antibody is expected to recognize isoform 1, but not isoform 2, of SMUG1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro179 of human SMUG1 protein. Background The base excision repair (BER) pathway corrects small base lesions in DNA due to oxidation, deamination, or misincorporation of dUTP instead of dTTP during DNA replication. These bases are removed by DNA glycosylases, resulting in abasic sites that are then repaired through short- or long-patch BER (1,2). Single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1) removes uracil and oxidized pyrimidines from DNA. SMUG1 expression is important for genome stability, and high expression of SMUG1 is correlated with favorable prognosis in human cancer (3). Knockdown or knockout of SMUG1 results in decreased proliferation, as well as increased DNA damage and apoptosis (4). Alternate Names FDG; HMUDG; MGC104370; Single-strand selective monofunctional uracil DNA glycosylase; single-strand-selective monofunctional uracil-DNA glycosylase 1; SMUG1; UNG3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG