CST, 23689SF, IL-1 beta (D6D6T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IL1 beta mouse. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, IL-1 beta (D6D6T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #23689) is ready to ship. Product Usage Information This product is the carrier free version of product #31202. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 31202 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IL-1 beta (D6D6T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total IL-1β protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to mouse IL-1β protein. Background Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. Alternate Names IL-; IL-1 beta; Il-1b; IL-1beta; Il1b; interleukin 1 beta; Interleukin-1 beta; OTTMUSP00000016525 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 17, 31 Source/Isotype: Rabbit IgG