CST, 2369S, Villin-1 (R814) Antibody

Polyclonal Antibody for studying Villin1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Villin-1 (R814) Antibody (CST #2369) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Villin-1 (R814) Antibody recognizes endogenous levels of total villin-1 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human villin-1. Antibodies are purified using protein A and peptide affinitty chromatography. Background Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli (1,2). Phosphorylation of villin at Tyr60, 81, and 256 may be involved in the regulation of cell migration (3). Expression of villin is increased in colorectal cancers (4), and villin-1 function appears to be involved in progressive cholestasis and hepatic failure (5). Alternate Names D2S1471; VIL; VIL1; VILI; Villin; villin 1; Villin-1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 95 SOURCE: Rabbit