CST, 23706S, Phospho-alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Synuclein-alpha (Ser129) phosphate. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Antibody (CST #23706) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 87281 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity Phospho-alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Antibody recognizes endogenous levels of α-Synuclein protein only when phosphorylated at Ser129. Specificity of nuclear staining is unclear but consistent with previous publications (4). Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser129 of human α-Synuclein protein. Background α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1). Various research studies have shown that phosphorylation of α-Synuclein at Ser129 is a highly toxic event causing degeneration of dopaminergic neurons associated with Parkinson's disease. This is proposed to occur through increased misfolding, aggregation, and accumulation of α-Synuclein phosphorylated at this site (2). GSK-3β is one of several kinases that has been reported to phosphorylate α-Synuclein at Ser129 (3). Alternate Names Alpha-synuclein; alpha-synuclein, isoform NACP140; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); SYUA; truncated alpha synuclein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG