CST, 2372S, beta-Gal (14B7) Mouse Monoclonal Antibody

Monoclonal Antibody for studying beta-galactosidase bacteria. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, beta-Gal (14B7) Mouse Monoclonal Antibody (CST #2372) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:3500 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity beta-Gal (14B7) Mouse Monoclonal Antibody detects transfected beta-galactosidase fusion proteins. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant beta-galactosidase protein. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Eukaryotic genes can be cloned into E. coli beta-galactosidase (lacZ) gene (1-3). This results in the expression of a fusion protein containing the desired protein with a beta-gal tag. An antibody to this tag can then be used to isolate the fusion protein or detect its expression within cells or tissues. Alternate Names b0344; beta-D-galactosidase; beta-gal; Beta-galactosidase; JW0335; lactase; lacZ Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1