CST, 23757T, Iba1/AIF-1 (E4O4W) Horse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying AIF1/Iba1. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Iba1/AIF-1 (E4O4W) Horse Chimeric Monoclonal Antibody (CST #23757) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Iba1/AIF-1 (E4O4W) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total Iba1/AIF-1 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification This recombinant chimeric antibody is engineered from Iba1/AIF-1 (E4O4W) XP  Rabbit mAb #17198 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Iba1/AIF-1 (E4O4W) XP  Rabbit mAb #17198, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala139 of human Iba1/AIF-1 protein. Background Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is an evolutionarily conserved cytoplasmic calcium binding protein containing a central pair of EF-hand calcium binding motifs (1,2). Iba1/AIF-1 was originally cloned from activated macrophages in human atherosclerotic allogenic heart grafts undergoing chronic transplant rejection as well as from rat monocytes (3,4). Its function is not very well understood, but Iba1/AIF-1 expression is upregulated in response to interferon-gamma and, therefore, could modulate macrophage-dependent immune response (3). As an F-actin-binding protein, Iba1/AIF-1 may function to remodel the actin cytoskeleton and contribute to morphological changes that correlate with various microglial/macrophage states (5). Iba1/AIF-1 is also uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (6). Alternate Names AIF-1; AIF1; Allograft inflammatory factor 1; G1; IBA 1; IBA-1; IBA1; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT-1; IRT1; Protein G1 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous Source/Isotype: Horse chimeric IgG4