CST, 23957S, CCL18 (F9S6V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CCL18. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, CCL18 (F9S6V) Rabbit Monoclonal Antibody (CST #23957) is ready to ship. Product Usage Information Western Blotting: 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity CCL18 (F9S6V) Rabbit Monoclonal Antibody recognizes endogenous levels of total CCL18 protein. This antibody does not cross-react with CCL3/MIP-1α or CCL4 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCL18 protein. Background C-C motif chemokine ligand 18 (CCL18, PARC, MIP-4, AMAC-1, DC-CK1) is a member of the CC chemokine family involved in signaling and coordinating immune responses (1-3). CCL18 is a secreted protein, primarily expressed by macrophages, monocytes, and dendritic cells (1-3). Th2-associated anti-inflammatory cytokines such as IL-4, IL-13, and IL-10 can polarize macrophages into an M2 phenotype, which induces CCL18 expression (3,4). In cancer, tumor-associated macrophages (TAMs) may secrete large amounts of CCL18, causing an immunosuppressive tumor microenvironment, which can lead to cancer immune evasion (5). Alternate Names Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1; C-C motif chemokine 18; C-C motif chemokine ligand 18; CC chemokine ligand 18; CC chemokine PARC; CCL18; CCL18(1-68); CCL18(3-69); CCL18(4-69); chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); chemokine (C-C), dendritic; CKb7; DC-CK1; DCCK1; Dendritic cell chemokine 1; Macrophage inflammatory protein 4; MIP-4; MIP4; PARC; Pulmonary and activation-regulated chemokine; SCYA18; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated; Small-inducible cytokine A18 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 9 Source/Isotype: Rabbit IgG