CST, 2400T, Synip (C51G6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Synip. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Synip (C51G6) Rabbit Monoclonal Antibody (CST #2400) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Synip (C51G6) Rabbit Monoclonal Antibody detects endogenous levels of total Synip protein. Species Reactivity: Human, Mouse, Rat Source / Purification Synip (C51G6) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to the sequence of human Synip. Background Insulin binds to and activates its receptor and initiates a signaling cascade that eventually induces the translocation of the Glut4 glucose transporter from its intracellular locations to the plasma membrane. Initiating this pathway facilitates glucose uptake in fat and skeletal muscle cells (1). Synip and Syntaxin 4 are two proteins thought to be involved in the recruitment of Glut4-containing vesicles to plasma membrane (2,3). Synip associates with Syntaxin 4 when insulin is absent. Insulin signaling triggers the dissociation of the two proteins and allows Syntaxin 4 to complex with VAMP2, which is essential for Glut4 translocation to plasma membrane (2-4). Overexpression of a dominant-negative form of Synip prevents Glut4 from translocating to plasma membrane in response to insulin stimulation (3). Synip together with Syntaxin 4, therefore, regulates Glut 4 transport to plasma membrane. Alternate Names FLJ16496; MGC149829; MGC50337; STX4-interacting protein; STXB4; STXBP4; Synip; syntaxin 4 interacting protein; Syntaxin 4-interacting protein; syntaxin binding protein 4; Syntaxin-binding protein 4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG