CST, 24129S, Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRF-7 (Ser437/Ser438) phosphate mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit Monoclonal Antibody (CST #24129) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-IRF-7 (Ser437/438) (D6M2I) Rabbit Monoclonal Antibody recognizes endogenous levels of mouse IRF-7 protein when dually phosphorylated at Ser437 and Ser438. This antibody has a preference for Ser437 and can recognize single phosphorylation at this site. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser437/438 of mosue IRF-7 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response to viral infection (6-8). Like IRF-3, IRF-7 is regulated at multiple serine phosphorylation sites near its carboxyl terminus, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11). Alternate Names Interferon regulatory factor 7; IRF-7; Irf7; OTTMUSP00000029373 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG