CST, 24151SF, Tenascin C (E6N4L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Tenascin C. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Tenascin C (E6N4L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #24151) is ready to ship. Product Usage Information This product is the carrier free version of product #93029. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 93029 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Tenascin C (E6N4L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Tenascin C protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Tenascin C protein. Background Tenascin C is a large hexameric extracellular matrix glycoprotein that exhibits de-adhesive effects on cell-matrix interaction, enhancing cell proliferation and motility in most cell types. It is highly expressed in remodeling tissues during embryonic development and under pathological conditions in adults, and research studies have shown markedly increased expression in cancerous tissues (1,2). Tenascin C has been implicated in a variety of cellular processes relevant to atherosclerosis, including cell proliferation, migration, and apoptosis. Expression of Tenascin C is tightly controlled in adults and is upregulated in tissues undergoing wound healing (3). In development, the expression of Tenascin C is known to be associated with epithelial-mesenchymal transition (EMT) events, including gastrulation and formation of the neural crest, endocardial cushion, and secondary palate (1). Investigators have shown that Tenascin C is a key determinant of the tumor stroma and is involved in the initiation of tumorigenesis and progression to metastasis (2). Immature and mature astrocytes, radial glial cells, Schwann cells, and a subset of neurons express Tenascin C. Upon CNS trauma or exposure of neurons to excitotoxic agents, Tenascin C expression is upregulated by glial cells. Research studies have shown that Tenascin C is involved in guidance of migrating axons and neurons, synaptic plasticity, and neuronal regeneration, promoting spinal cord regeneration after injury (4). Alternate Names 150-225; Cytotactin; deafness, autosomal dominant 56; DFNA56; Glioma-associated-extracellular matrix antigen; GMEM; GP; GP 150-225; Hexabrachion; hexabrachion (tenascin); HXB; JI; MGC167029; Myotendinous antigen; Neuronectin; TENA; Tenascin; tenascin C; Tenascin-C; tenascin-C additional domain 1; tenascin-C isoform 14/AD1/16; TN; TN-C; TNC Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200, 240 Source/Isotype: Rabbit IgG