CST, 24161S, ZIP14/SLC39A14 (E3H7D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLC39A14. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, ZIP14/SLC39A14 (E3H7D) Rabbit Monoclonal Antibody (CST #24161) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity ZIP14/SLC39A14 (E3H7D) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZIP14/SLC39A14 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu282 of human ZIP14/SLC39A14 protein. Background Solute carrier (SLC) family member Zrt- and Irt-like protein 14 (ZIP14) is a transmembrane metal ion symporter encoded by the gene . ZIP14 functions as a plasma membrane transporter of zinc and manganese, and as an endosomal promoter of intracellular uptake of iron from transferrin (1). In liver, ZIP14 localizes to the basolateral membrane of hepatocytes, transporting manganese from the blood for eventual delivery to the bile duct (2). In pancreatic beta cells, expression is glucose-sensitive and plays a key role in zinc-dependent insulin secretion (3). The reported ZIP14 L441R mutation (L438R in mice) disrupts plasma membrane presentation of the transporter, causing intracellular accumulation of zinc in osteoblasts, characterized by excessive bone overgrowth (4). mutation or knockdown can cause manganese dyshomeostasis, resulting in childhood-onset dystonia-Parkinsonism (5). The activity of liver-expressed ZIP14/SLC39A14, intestinal SLC39A10, and blood-brain barrier ZIP8/SLC39A8 all contribute to manganese homeostasis during neuronal development (6,7). Alternate Names cig19; HCIN; HMNDYT2; KIAA0062; LIV-1 subfamily of ZIP zinc transporter 4; LZT-Hs4; Metal cation symporter ZIP14; NET34; S39AE; SLC39A14; solute carrier family 39 (metal ion transporter), member 14; solute carrier family 39 (zinc transporter), member 14; Solute carrier family 39 member 14; Zinc transporter ZIP14; ZIP-14; ZIP14; Zrt- and Irt-like protein 14; Zrt-, Irt-like protein 14 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55-65 Source/Isotype: Rabbit IgG