CST, 24179T, FAM122A (E3X4R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FAM122A. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FAM122A (E3X4R) Rabbit Monoclonal Antibody (CST #24179) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FAM122A (E3X4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total FAM122A protein. By western blot, this antibody may detect a band of unknown origin at approximately 80 kDa in some samples. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala132 of human FAM122A protein. Background FAM122A is an inhibitor of serine/threonine-protein phosphatase 2A (PP2A) activity and stability (1). FAM122A promotes the growth of hepatocellular carcinoma (HCC) and acute myeloid leukemia (AML) cells, and increased expression of FAM122A has been reported in HCC and AML cancers (2,3). Chk1 phosphorylates FAM122A at Ser37, inactivating FAM122A, and therefore activating PP2A. PP2A promotes G2/M checkpoint activation through dephosphorylation of the CDK1 inhibitory kinase WEE1. CRISPR knockout of FAM122A confers resistance to inhibitors of Chk1 and ATR in lung cancer cell lines. Expression of FAM122A may serve as a biomarker for sensitivity to Chk1 inhibitors, which are under investigation for the treatment of non-small cell lung cancer (NSCLC) and other cancers (4). Alternate Names C9orf42; FAM122A; family with sequence similarity 122A; MGC17347; PABIR family member 1; PBIR1; PPP2R1A-PPP2R2A-interacting phosphatase regulator 1; Protein FAM122A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG