CST, 24232T, Caspase-1 (E2Z1C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Casp1 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-1 (E2Z1C) Rabbit Monoclonal Antibody (CST #24232) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Caspase-1 (E2Z1C) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse caspase-1 protein. This antibody detects pro-caspase-1 and the p10 subunit of activated caspase-1. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse caspase-1 protein. Background Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε, and ζ (6). Most caspase-1 isoforms (α, β, γ, and δ) produce products between 30-48 kDa and induce apoptosis upon overexpression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis, and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7). Alternate Names Casp; CASP-1; Casp1; caspase 1; Caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; Caspase1; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; Il1; Il1bc; interleukin 1 beta-converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 48, 10 Source/Isotype: Rabbit IgG