CST, 2425S, eIF4A (F52) Antibody

Polyclonal Antibody for studying eIF4A2/eIF4A1/eIF4A3. Validated for Western Blotting. Highly specific and rigorously validated in-house, eIF4A (F52) Antibody (CST #2425) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity eIF4A (F52) Antibody detects endogenous levels of total eIF4A1, eIF4A2 and eIF4A3 proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe52 of human eIF4A. Antibodies are purified by protein A and peptide affinity chromatography. Background A variety of factors contribute to the important biological event of translation initiation. The eukaryotic initiation factor 4F (eIF4F) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signaling in a translation-independent manner in (4,5). Alternate Names ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-1; ATP-dependent RNA helicase eIF4A-2; ATP-dependent RNA helicase eIF4A-3; BM-010; DDX2A; DDX2B; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; DKFZp686O16189; EIF-4A; eIF-4A-I; eIF-4A-II; eIF-4A-III; EIF4A; eIF4A-I; eIF4A-II; eIF4A-III; EIF4A1; EIF4A2; EIF4A3; eIF4AIII; EIF4F; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4A-II; Eukaryotic initiation factor 4A-III; Eukaryotic initiation factor 4A-III, N-terminally processed; Eukaryotic initiation factor 4A-like NUK-34; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A, isoform 1; eukaryotic translation initiation factor 4A, isoform 2; eukaryotic translation initiation factor 4A, isoform 3; eukaryotic translation initiation factor 4A1; eukaryotic translation initiation factor 4A2; eukaryotic translation initiation factor 4A3; Fal1; hNMP 265; IF4A1; IF4A2; IF4A3; KIAA0111; MGC10862; MUK34; NMP 265; NMP265; Nuclear matrix protein 265; NUK34; RCPS Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 48 SOURCE: Rabbit