CST, 24317T, MMP-9 (E7N3Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MMP9 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MMP-9 (E7N3Y) Rabbit Monoclonal Antibody (CST #24317) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MMP-9 (E7N3Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total MMP-9 protein. This antibody detects both mature MMP-9 (84 kDa) and proMMP-9 (full-length 92 kDa). Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to the amino terminus of mouse MMP-9 protein. Background The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (5). Alternate Names 92 kDa gelatinase; 92 kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; 92kDa gelatinase; 92kDa type IV collagenase; AW743869; B/MMP; B/MMP9; Clg4; Clg4b; collagenase type IVB; Gel B; Gelatinase B; GELB; matrix metallopeptidase 9; matrix metalloproteinase 9; Matrix metalloproteinase-9; MMP-; MMP-9; Mmp9; OTTMUSP00000001145; pro-MMP-9 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 84, 92 Source/Isotype: Rabbit IgG