CST, 2439T, ATGL (30A4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATGL. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, ATGL (30A4) Rabbit Monoclonal Antibody (CST #2439) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 89633 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity ATGL (30A4) Rabbit Monoclonal Antibody detects endogenous levels of total ATGL protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL. Background Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6). Alternate Names 1110001C14Rik; Adipose triglyceride lipase; ATGL; Calcium-independent phospholipase A2; Desnutrin; DKFZp667M109; FP17548; IPLA2-zeta; iPLA2zeta; mutant patatin-like phospholipase domain containing 2; patatin like phospholipase domain containing 2; patatin-like phospholipase domain containing 2; Patatin-like phospholipase domain-containing protein 2; PEDF-R; Pigment epithelium-derived factor; PLPL2; PNPLA2; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS-2.2; TTS2; TTS2.2 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 54 Source/Isotype: Rabbit IgG