CST, 2442S, HSP27 Antibody

Polyclonal Antibody for studying HSP27 mouse. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, HSP27 Antibody (CST #2442) is ready to ship. Product Usage Information Western Blotting: 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:25 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity HSP27 Antibody detects endogenous levels of total HSP27 protein. This antibody does not cross-react with other heat shock proteins. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to rat HSP27. Antibodies are purified by protein A and peptide affinity chromatography. Background Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). Alternate Names 27kDa; Growth-related 25 kDa protein; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kDa protein 1; heat shock protein 1; Heat shock protein beta-1; heat shock protein, 25 kDa; Hsp; HSP 25; HSP 27; Hsp25; Hsp27; HspB1; OTTMUSP00000028918; p25; truncated hsp25 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 27 SOURCE: Rabbit