CST, 24470SF, MUC5B (E4Y5Z) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MUC5B. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, MUC5B (E4Y5Z) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #24470) is ready to ship. Product Usage Information This product is the carrier free version of product #74978. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 74978 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MUC5B (E4Y5Z) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MUC5B protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MUC5B protein. Background Mucins are a family of macromolecules that line and protect the respiratory epithelium from microbes and pollutants in the local environment. Of the family members that are known to date, some are produced in a cell type and tissue-specific manner, suggesting distinct biological roles for members. Some members polymerize after secretion to form gel-like substances that coat the epithelial layer. MUC5AC and MUC5B are family members that polymerize in this manner. Others do not polymerize, and others yet, have a transmembrane domain and remain physically attached to the epithelia (1). While it is known that mucins are protective to the respiratory epithelium, it has been reported that changes in expression of mucins are associated with several forms of lung disease, such as cystic fibrosis, COPD, asthma, pulmonary fibrosis, and others (1-4). Multiple studies have shown that one of the largest known risks of developing lung fibrosis is linked to a genetic variant of MUC5B (5,6). Alternate Names Cervical mucin; cervical mucin MUC5B; high molecular weight mucin; High molecular weight salivary mucin MG1; MG1; MUC-5B; MUC5; MUC5B; MUC9; mucin 5, subtype B, tracheobronchial; mucin 5B, oligomeric mucus/gel-forming; Mucin-5 subtype B, tracheobronchial; Mucin-5B; mucin, salivary (high molecular weight); Sublingual gland mucin Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG