CST, 2456S, PSMA3 Antibody

Polyclonal Antibody for studying PSMA3. Validated for Western Blotting. Highly specific and rigorously validated in-house, PSMA3 Antibody (CST #2456) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PSMA3 Antibody detects endogenous levels of total PSMA3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val228 of human PSMA3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of α subunits (called PSMAs), and the two inner layers are identical rings composed of β subunits. While the catalytic sites are located on the β rings (1-3), the α subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different α and ten different β proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades polyubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9). Alternate Names HC8; Macropain subunit C8; MGC12306; MGC32631; Multicatalytic endopeptidase complex subunit C8; proteasome (prosome, macropain) subunit, alpha type, 3; proteasome 20S subunit alpha 3; Proteasome component C8; proteasome subunit alpha 3; Proteasome subunit alpha type-3; proteasome subunit alpha7; proteasome subunit C8; PSA3; PSC3; PSC8; PSMA3; testicular secretory protein Li 43 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit