CST, 24573SF, ITK (E4X7M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Itk. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, ITK (E4X7M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #24573) is ready to ship. Product Usage Information This product is the carrier free version of product #77215. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 77215 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ITK (E4X7M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ITK protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ITK protein. Background Interleukin-2 inducible T cell kinase (Itk, Emt, or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk, and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain, and an SH3 and SH2 domain followed by a carboxy-terminal kinase domain (1). Tec, Rlk, and Itk are expressed in T cells and activated in response to T cell receptor (TCR) engagement. Data demonstrate that Itk functions in signal transduction downstream of TCR and activates PLCgamma1 and Erk. Lck directly activates Itk through phosphorylation in the conserved activation loop at Tyr511, and furthermore, Itk is autophosphorylated in the SH3 domain at Tyr180. Itk-Y180F is still capable of phosphorylating PLCgamma1 in contrast to Itk-Y511F, which has lost that function (2-3). Itk -/- mice show reduced lung inflammation, eosinophil infiltration, and mucous production in response to allergic asthma induction. Thus, Itk could become a desirable target for anti-asthmatic treatments (4). Alternate Names EMT; homolog of mouse T-cell itk/tsk; IL-2-inducible T-cell kinase; IL2 inducible T cell kinase; IL2-inducible T-cell kinase; Interleukin-2-inducible T-cell kinase; ITK; Kinase EMT; LPFS1; LYK; MGC126257; MGC126258; PSCTK2; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 72 Source/Isotype: Rabbit IgG