CST, 24588S, NUP62 (E4Y5P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NUP62. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, NUP62 (E4Y5P) Rabbit Monoclonal Antibody (CST #24588) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity NUP62 (E4Y5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total NUP62 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly170 of human NUP62 protein. Background The nuclear pore complex (NPC) is a multi-subunit protein channel that spans the nuclear envelope and is responsible for the nucleocytoplasmic trafficking of RNA, proteins, and ribonucleoproteins (1,2). Nucleoporin p62 (NUP62) is a ubiquitously-expressed 62 kDa protein that is an essential component of the NPC (3). NUP62 expression is increased in aggressive, lethal prostate cancer, and knockdown of NUP62 expression reduces cancer cell proliferation and colony formation in soft agar (4). NUP62 is also highly expressed in squamous cell carcinomas (SCCs), and depletion of NUP62 in SCC cells results in reduced proliferation and enhanced differentiation via regulation of p63 nucleo-cytoplasmic transport (5). Interestingly, NUP62 also plays a role in mitotic cell cycle progression after disassembly of the nuclear envelope and NPCs. Indeed, knockdown of NUP62 induces G2/M phase arrest, mitotic cell death, and abnormal centrosome assembly (6). A missense mutation of Q391P in NUP62 is believed to cause infantile bilateral striatal necrosis (IBSN), a rare and fatal autosomal recessive disease (7). Alternate Names 62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; IBSN; MGC841; Nuclear pore glycoprotein p62; nucleoporin 62; nucleoporin 62kD; nucleoporin 62kDa; Nucleoporin Nup62; NUP62; p62; SNDI Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG