CST, 24773T, Etk/BMX (D6J1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Etk/BMX. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Etk/BMX (D6J1S) Rabbit Monoclonal Antibody (CST #24773) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Etk/BMX (D6J1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total Etk/BMX protein. This antibody does not cross-react with Tec, Itk or Btk. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Etk/BMX protein. Background Etk, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family (1). It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways (2). Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo (3). In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40 (4). Alternate Names BMX; BMX non-receptor tyrosine kinase; Bone marrow tyrosine kinase gene in chromosome X protein; BTK-like on X chromosome; Cytoplasmic tyrosine-protein kinase BMX; Epithelial and endothelial tyrosine kinase; ETK; Etk/Bmx cytosolic tyrosine kinase; NTK38; NTK38 tyrosine kinase; PSCTK2; PSCTK3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG