CST, 24780S, Bcl-xL (54H6) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying BCL-xL. Validated for Western Blotting. Highly specific and rigorously validated in-house, Bcl-xL (54H6) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #24780) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Bcl-xL (54H6) Rabbit Monoclonal Antibody (HRP Conjugate) detects endogenous levels of total Bcl-xL protein. This antibody conjugate does not cross-react with other Bcl-2 family members. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL. Background Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents, such as paclitaxel, vinblastine, and nocodazole (4,5). Alternate Names Apoptosis regulator Bcl-X; B2CL1; Bcl-2-like protein 1; Bcl-X; bcl-xL; BCL-XL/S; bcl-xS; BCL2 like 1; Bcl2-L-1; BCL2-like 1; BCL2L; BCL2L1; BCLX; BCLXL; BCLXS; DKFZp781P2092; PPP1R52; protein phosphatase 1, regulatory subunit 52 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG