CST, 2480S, eIF5 Antibody

Polyclonal Antibody for studying eIF5. Validated for Western Blotting. Highly specific and rigorously validated in-house, eIF5 Antibody (CST #2480) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity eIF5 Antibody detects endogenous levels of total eIF5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence surrounding Val417 of human eIF5. Antibodies are purified by protein A and peptide affinity chromatography. Background Eukaryotic translation initiation factor 5 (eIF5) is crucial for the assembly of translation initiation complex and plays an important role in protein synthesis (1). eIF5 interacts with the 43S initiation complex to stimulate hydrolysis of GTP bound to eIF2 (1-3). Studies suggest that eIF5 functions as the GTPase-activating protein (GAP) in the hydrolysis of GTP-bound eIF2 (4,5). This hydrolysis leads to the release of initiation factors from the 40S ribosomal subunit, which is a necessary step in the formation of the 80S initiation complex (1). Alternate Names eIF-5; EIF-5A; EIF5; Eukaryotic translation initiation factor 5; IF5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 58 SOURCE: Rabbit