CST, 24840S, Cystatin C (D6U3E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CST3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cystatin C (D6U3E) Rabbit Monoclonal Antibody (CST #24840) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Cystatin C (D6U3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total cystatin C protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human cystatin C protein. Background Cystatin C (cystatin-3, CST3) belongs to the cystatin type 2 superfamily of cysteine peptidase inhibitors (1). The 146 amino acid cystatin C precursor protein contains a 26 residue, amino-terminal secretory signal sequence. The mature 120 amino acid cystatin C protein contains two disulfide bridges (2,3). Cystatin C is secreted in body fluids and is a marker of kidney (4) and cardiovascular (5) dysfunction. Research studies report of changes in cystatin C levels in the cerebral spinal fluid as well as in specific neuronal cell populations in a number of neurodegenerative diseases (6-8). Interestingly, experimental evidence suggests that cystatin C has protective effects against neurodegeneration, such as inhibition of amyloid-β oligomerization and fibril formation (9,10), induction of autophagy (11), induction of neurogenesis (12), and inhibition of cysteine proteases whose activity has been associated with several neurodegenerative diseases (13). Alternate Names ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C; Cystatin-3; Cystatin-C; CYTC; epididymis secretory protein Li 2; Gamma-trace; HEL-S-2; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG