CST, 2484S, Phospho-PLCbeta3 (Ser1105) Antibody

Polyclonal Antibody for studying PLCb3 (Ser1105) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-PLCbeta3 (Ser1105) Antibody (CST #2484) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PLCbeta3 (Ser1105) Antibody detects endogenous levels of PLCbeta3 only when phosphorylated at serine 1105. This antibody does not cross-react with phosphorylated PLCbeta1, PLCbeta2, PLCbeta4 or other PLCs. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding top residues surrounding Ser1105 of human PLCbeta3. Antibodies are purified by protein A and peptide affinity chromatography. Background Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1248 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8). Alternate Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3; FLJ37084; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-beta-3; phospholipase C beta 3; Phospholipase C-beta-3; phospholipase C, beta 3 (phosphatidylinositol-specific); PLC beta 3; PLC-beta-3; PLCB3; SMDCD Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit