CST, 2485S, VEGF Receptor 3 Antibody

Polyclonal Antibody for studying VEGFR3. Validated for Western Blotting. Highly specific and rigorously validated in-house, VEGF Receptor 3 Antibody (CST #2485) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VEGF Receptor 3 Antibody detects transfected levels of VEGFR-3. This antibody does not cross-react with VEGFR-1 and -2. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg961 of human VEGFR-3. Antibodies are purified by protein A and peptide affinity chromatography. Background Vascular endothelial growth factor receptor 3 (VEGFR3) is a 195 kDa membrane receptor tyrosine kinase. VEGF receptors are characterized by the presence of seven extracellular immunoglobulin (Ig)-like domains followed by a membrane-spanning domain and a conserved intracellular tyrosine kinase domain (1). VEGF receptor 3 expression is largely restricted to adult lymphatic endothelium and is thought to control lymphangiogenesis (1,2). Binding of VEGF-C/VEGF-D to VEGFR3 results in transphosphorylation of tyrosine residues in its intracellular domain, recruitment of signaling molecules and activation of ERK1/2 and Akt signaling cascades (1,3). Alternate Names CHTD7; Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4; FLT-4; FLT4; FLT41; fms related receptor tyrosine kinase 4; fms related tyrosine kinase 4; Fms-like tyrosine kinase 4; fms-related tyrosine kinase 4; LMPH1A; LMPHM1; PCL; primary congenital lymphedema; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; VEGF receptor-3; VEGFR-3; VEGFR3; VGFR3 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 195 SOURCE: Rabbit